tryple 10 (Fisher Scientific)

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Fisher Scientific tryple 10
Tryple 10, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tryple/tryple/us12654167-241-12-14
Average 86 stars, based on 1 article reviews

tryple 10 - by Bioz Stars, 2026-10

86/100 stars

Images

Single Cell:

Article Title: Immune Cell Modulation of Patient-Matched Organoid Drug Response in Precision Cancer Medicine Platform.
Article Snippet: .. Then, 1 mL of TrypLE (Fischer Scientific) was added to the cell pellet to dissociate the PDOs into a single-cell suspension. .. PBS was added, centrifugation was repeated, and the cells were plated by dividing the cell pellet into two wells in a 96-well V-bottom plate (Sigma-Aldrich).

Article Title: Efficient multi-kilobase knock-ins in mice and cell lines using CRISPR/Cas9 and rAAV donors with unbiased whole-genome characterization by LOCK-seq
Article Snippet: Nucleofection iPSCs were seeded into 500 μl of mTeSR Plus medium supplemented with 1x CloneR2 (STEMCELL Technologies, cat. #100-0691) for 24 h, and the medium was changed to mTeSR Plus without CloneR2. .. To generate clonal edited lines, pools were single-cell dissociated with 0.25% trypsin for N2a, BV2, and HEK293T cells or 0.75× TrypLE (Fisher Scientific, cat. #12-604-013) for iPSCs, and single cells were sorted into 96-well tissue culture plates on a Sony SH800 cell sorter. .. N2a cells were sorted into complete medium supplemented with 10 μM ROCK inhibitor (MilliporeSigma cat. #SCM075) and 1 mM sodium pyruvate (ThermoFisher, cat. #11360070), while HEK293T cells were sorted into 50% conditioned medium, and iPSCs were sorted into mTeSR Plus supplemented with 1x CloneR2.

Article Title: Immune Cell Modulation of Patient-Matched Organoid Drug Response in Precision Cancer Medicine Platform
Article Snippet: .. Then, 1 mL of TrypLE (Fischer Scientific) was added to the cell pellet to dissociate the PDOs into a single-cell suspension. .. PBS was added, centrifugation was repeated, and the cells were plated by dividing the cell pellet into two wells in a 96-well V-bottom plate (Sigma-Aldrich).

Suspension:

Incubation:

Article Title: Multi-gene biomarkers reveal spatial organization and subpopulation-specific damage response in intrahepatic biliary epithelial cells
Article Snippet: .. Supernatant was carefully discarded by pipetting and organoid pellet was resuspended in 1mL TrypLE (Fisher Scientific; 12605010) + 10μM Y-27632 (Selleck Chemicals; S1049) and incubated at 37°C for four minutes. ..

Article Title: Multi-gene biomarkers reveal spatial organization and subpopulation-specific damage response in intrahepatic biliary epithelial cells
Article Snippet: .. For single cell isolation, samples were resuspended with 3mL TrypLE (Fisher Scientific; 12605010) + 10μM Y-27632 (Selleck Chemicals; S1049) and incubated at 37°C for 12min. ..

Single-cell Isolation:

Article Title: Multi-gene biomarkers reveal spatial organization and subpopulation-specific damage response in intrahepatic biliary epithelial cells
Article Snippet: .. For single cell isolation, samples were resuspended with 3mL TrypLE (Fisher Scientific; 12605010) + 10μM Y-27632 (Selleck Chemicals; S1049) and incubated at 37°C for 12min. ..

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